|The Neuroscience Area is centered around a large fully multimedia equipped teaching laboratory with four individual workstations for use in the neuroscience course laboratories. In addition to the role as a teaching laboratory, the neuroscience area can accommodate a lecture class of up to 20 students.|
Four independent student electrophysiological set-ups with dissecting scopes can be used simultaneously in the main neuroscience laboratory area.
There are dedicated chemical storage and chemical preparation areas as well as a histology and surgery suite equipped with computers, microscopes, and surgical apparatus. Two externally vented fume hoods provide protection when working with hazardous or volatile chemicals.
The Gustatory Behavioral Lab
Dr. Pittman's gustatory behavioral laboratory allows students to measure the behavioral responses of rats and mice to various taste stimuli during brief-access or long-duration testing sessions.
The Davis MS-160 behavioral rig (shown left) allows automated presentations of up to 16 different taste
stimuli while collecting measurements of animals' behavioral responses at a resolution of 1 millisecond.
Data analysis programs include examination of cumulative licks, latency until first lick, interlick interval, and rate of licking.
The Davis MS-160 uses a customized computer program to control the order of stimuli, the time between each stimulus, and the length of the stimulus presentation.
The acoustic isolation chamber housing the Davis Rig contains air circulation fans, a white noise generator, and a web based camera to allow observation of the animal during testing.
View a video clip of a rat responding in the Davis Rig.
The shutter in the open position allows
access to one of sixteen bottle spouts.
A rat waits for the shutter to open
presenting a taste stimulus.
The AC-108 is an 8-channel lickometer capable of recording the licking behavior of rats for up to 24 hours. The 8 lickometers can be installed in single cages allowing testing of a single solution for 8 rats at a time or the lickometers can be installed 2 per cage to allow 2-bottle data collection for 4 rats at a time. The time of each lick made by a rat is recorded with a 1 microsecond resolution. Custom software allows microanalysis of the licking behavior. The microanalysis can be used to identify licking behaviors associated with taste-mediated cues such as lick rate and number of licks within a burst or licking behaviors typically associated with post-ingestive cues such as licks per meal and meal durations.
View a video clip of a rat being tested in the AC-108.
The Electrophysiological Recording Lab
In the spring of 2005, a state-of-the-art electrophysiological recording rig was added to the neuroscience laboratory. The rig is equipped with a floating tabletop, homeothermic temperature regulation, cautery, air turbine drill, aspirator, surgical tools, dissecting scope with camera, microdrive manipulator, differential amplifier, micro1401 & spike digital acquisition system, a DELL CPU, 19" LCD screen with PIP for CPU & scope camera dual display, and a custom stimulus delivery system.
The system is capable of recording integrated whole nerve responses and single nerve fiber responses as well as multi-unit and single-unit neuron responses in peripheral ganglion or the central nervous system.
|ASSOCIATED NEUROSCIENCE FACILITIES: |
Psychology Behavioral Processes Laboratory
Animal Husbandry Facilities
Return to the main Program in Neuroscience website.